Highly Induced DNA Recombination Mediated by Membrane Permeabilized Recombinant Cre Protein in Mouse Primary Cells
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概要
- 論文の詳細を見る
To establish efficient induction of Cre mediated DNA recombination in primary cells, mouse embryonic fibroblast, keratinocyte, and primary preosteoblast, we tested various recombinant Cres by fusing of protein transduction domain in human immunodeficiency virus (HIV) transactivator of transcription (TAT-PTD) to the N- and/or C-terminus. HTC, modified Cre with PTD at the N-terminus, achieved the highest activity of DNA recombination for those primary cells.
- 社団法人 日本農芸化学会の論文
- 2007-03-23
著者
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Hidema Shizu
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University
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Maruyama Takamitsu
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University
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Kato Shigeki
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University
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NISHIMORI Katsuhiko
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University
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Kato Shigeki
Department Of Internal Medicine Tsu National Hospital
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Kato Shigeki
Department Of Molecular And Cell Biology Graduate School Of Agricultural Science Tohoku University
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Nishimori Katsuhiko
Department Of Molecular And Cell Biology Graduate School Of Agricultural Science Tohoku University
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Maruyama Takamitsu
Department Of Molecular And Cell Biology Graduate School Of Agricultural Science Tohoku University
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Hidema Shizu
Department Of Molecular And Cell Biology Graduate School Of Agricultural Science Tohoku University
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Kato Shigeki
Department of Chemistry, College of Arts and Sciences, The University of Tokyo
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