Usefulness of Heterologous Promoters in the Pseudozyma flocculosa Gene Expression System

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概要

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• The basidiomycetous fungus Pseudozyma flocculosa represents a promising new host for the expression of complex recombinant proteins. Two novel heterologous promoter sequences, the Ustilago maydis glyceraldehyde-3-phosphate dehydrogenase (GPD) and Pseudozyma tsukubaensis α-glucosidase promoters, were tested for their ability to provide expression in P. flocculosa. In liquid medium, these two promoters produced lower levels of intracellular green fluorescent protein (GFP) as compared to the U. maydis hsp70 promoter. However, GPD and α-glucosidase sequences behaved as constitutive promoters whereas the hsp70 promoter appeared to be morphology-dependent. When using the hsp70 promoter, the expression of GFP increased proportionally to the concentration of hygromycin in the culture medium, indicating possible induction of the promoter by the antibiotic. Optimal solid-state culture conditions were designed for high throughput screening of hygromycin-resistant transformants with the hsp70 promoter in P. flocculosa.

• 社団法人 日本農芸化学会の論文

著者

• Belanger Richard

  Department De Phytologie Universite Laval

• AVIS Tyler

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• ANGUENOT Raphaël

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• NEVEU Bertrand

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• BOLDUC Sébastien

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• ZHAO Yingyi

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• CHENG Yali

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• LABBÉ Caroline

  Department of Phytology, Horticulture Research Centre, Envirotron, University Laval

• BELZILE François

  Department of Phytology, C.-E. Marchand, University Laval

関連論文

• Usefulness of Heterologous Promoters in the Pseudozyma flocculosa Gene Expression System
• Recombinant Protein Secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a Novel Signal Peptide
• Usefulness of Heterologous Promoters in the Pseudozyma flocculosa Gene Expression System
• Novel Butyrolactones with Antifungal Activity Produced by Pseudomonas aureofaciens Strain 63-28

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