Recombinant Protein Secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a Novel Signal Peptide
スポンサーリンク
概要
- 論文の詳細を見る
Secretion of recombinant proteins aims to reproduce the correct posttranslational modifications of the expressed protein while simplifying its recovery. In this study, secretion signal sequences from an abundantly secreted 34-kDa protein (P34) from Pseudozyma flocculosa were cloned. The efficiency of these sequences in the secretion of recombinant green fluorescent protein (GFP) was investigated in two Pseudozyma species and compared with other secretion signal sequences, from S. cerevisiae and Pseudozyma spp. The results indicate that various secretion signal sequences were functional and that the P34 signal peptide was the most effective secretion signal sequence in both P. flocculosa and P. antarctica. The cells correctly processed the secretion signal sequences, including P34 signal peptide, and mature GFP was recovered from the culture medium. This is the first report of functional secretion signal sequences in P. flocculosa. These sequences can be used to test the secretion of other recombinant proteins and for studying the secretion pathway in P. flocculosa and P. antarctica.
- 社団法人 日本農芸化学会の論文
- 2008-12-23
著者
-
Belanger Richard
Department De Phytologie Universite Laval
-
BOLDUC Sébastien
Department of Phytology, Horticulture Research Centre, Envirotron, University Laval
-
LABBÉ Caroline
Department of Phytology, Horticulture Research Centre, Envirotron, University Laval
-
BELZILE François
Department of Phytology, C.-E. Marchand, University Laval
-
Cheng Yali
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Belanger Richard
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Avis Tyler
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Anguenot Raphael
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Zhao Yingyi
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Neveu Bertrand
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Labbe Caroline
Department Of Phytology Horticulture Research Centre Envirotron University Of Laval
-
Belzile Francois
Department Of Phytology C.-e. Marchand University Laval
関連論文
- Usefulness of Heterologous Promoters in the Pseudozyma flocculosa Gene Expression System
- Recombinant Protein Secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a Novel Signal Peptide
- Usefulness of Heterologous Promoters in the Pseudozyma flocculosa Gene Expression System
- Novel Butyrolactones with Antifungal Activity Produced by Pseudomonas aureofaciens Strain 63-28