Red Ginseng Acidic Polysaccharide (RGAP) in Combination with IFN-γ Results in Enhanced Macrophage Function through Activation of the NF-κB Pathway

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概要

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• This study examined the effects of red ginseng acidic polysaccharide (RGAP) on macrophage-mediated cytotoxicity towards murine melanoma B16 cells. RGAP alone had no effect on killing of tumor cells. RGAP treatment increased the production of interleukin-1 (IL-1), IL-6, and nitric oxide (NO) by macrophages, whereas tumor necrosis factor (TNF-α) and reactive oxygen species (ROS) production were not changed by RGAP. However, treatment of macrophages with a combination of RGAP and recombinant interferon-γ (rIFN-γ) enhanced killing of tumor cells. In addition, the combination treatment showed marked cooperative induction of IL-1, IL-6, TNF-α, and NO production. Electrophoretic mobility shift assay analysis revealed that treatment of macrophages with RGAP plus rIFN-γ induced the activation of the nuclear factor-kappa B (NF-κB) transcription factor. In agreement with this, the combination treatment resulted in increased NF-κB-p65 expression. The present results demonstrate synergistic effects on macrophage function of RGAP in combination with rIFN-γ, and suggest that NF-κB plays an important role in mediating these effects. These data also support the development of clinical studies of this combination.

• 社団法人 日本農芸化学会の論文

著者

• Moon Eun-Yi

  Department of Bioscience and Biotechnology, Sejong University

• PYO Suhkneung

  Division of Immunopharmacology, College of Pharmacy, Sung Kyun Kwan University

• CHOI Hye-Sook

  Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University

• KIM Kyung-Ho

  Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University

• SOHN Eunwha

  Department of Herbal Medicine Resources, Institute of Bioscience and Biotechnology, Kangwon National

• PARK Jong-Dae

  KT&G Central Research Institute

• KIM Byung-Oh

  Department of Applied Biology, Kyungpook National University

• RHEE Dong-Kwon

  Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University

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• Novel Sulfated Polysaccharide Derived from Red-Tide Microalga Gyrodinium impudicum strain KG03 with Immunostimulating Activity in vivo
• Red Ginseng Acidic Polysaccharide (RGAP) in Combination with IFN-γ Results in Enhanced Macrophage Function through Activation of the NF-κB Pathway
• Red Ginseng Acidic Polysaccharide (RGAP) in Combination with IFN-γ Results in Enhanced Macrophage Function through Activation of the NF-κB Pathway

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