A New Fluorometric Determination of Disulfides in Urine, Blood and Some Mammalian Tissues by KCN-NAM Method
スポンサーリンク
概要
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Disulfides, oxidized glutathione (GSSG) and cystine [(Cys)2], were determined with a new fluorescent reagent N-(9-acridinyl)maleimide via corresponding thiols which were derived by the reduction with potassium cyanide. Linear relations were obtained between the fluorescence intensities and the disulfides concentrations ranged from 5 pmol/ml to 5 nmol/ml of GSSG and 5 pmol/ml to 2.7 nmol/ml of (Cys)2, respectively. This method is 100-times more sensitive than the conventional colorimetric method. It enables determinations of disulfides in 2μl of urine, 6μl of blood and 0.6μg of tissues.
- 社団法人 日本農芸化学会の論文
著者
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Meguro Hiroshi
Laboratory Of Analytical Chemistry Faculty Of Agriculture Tohoku University
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TAKAHASHI Hiroaki
Laboratory of Analytical Chemistry, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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YOSHIDA Takanori
Laboratory of Analytical Chemistry, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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NARA Yasunori
Laboratory of Analytical Chemistry, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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TUZIMURA Katsura
Laboratory of Analytical Chemistry, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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