Some properties of amylase inhibitor A produced by Streptomyces sp. No. 280.
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概要
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Streptomyces sp. No. 280 produced several kinds of amylase inhibitors (amylase inhibitor A, B, B' and C). Two amylase inhibitors (designated as AI-A1 and AI-A2) were obtained from an amylase inhibitor A fraction by paper chromatography. AI-A1 inhibited muscle phosphorylase a much more than AI-A2 and was hydrolyzed by sweet potato β-amylase whereas AI-A2 was not. Both amylase inhibitors had a carbohydrate and were hydrolyzed by some kinds of amylases or acids. They lost their inhibitory activity against phosphorylase a after treatment with acids or hog pancreatic α-amylase, but they showed increased inhibitory activity toward porcine small intestinal sucrase. Both AI-A1 and AI-A2 were composed of glucose and a basic moiety which gave a positive ninhydrin reaction. The molecular weights of AI-A1 and AI-A2 were estimated to be approximately 1300-1500 by gel filtration on a Sephadex G-15 column. The nitrogen content of the amylase inhibitors was found to be about 1.3% by elementary analysis.
- 公益社団法人 日本農芸化学会の論文
著者
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NAKANO Hirofumi
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University