Spectrophotometric Determination Based on Difference Spectra of L-Ascorbic Acid in Plant and Animal Foods
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概要
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The difference spectra before and after oxidation of L-ascorbic acid (AsA) by ascorbate oxidase (EC 1.10.3.3) were measured using a recording spectrophotometer. A linear relationship was found between the peak height of difference spectra and the concentration of AsA. The AsA content in plant and animal foods was evaluated by this difference spectral method and was compared with that obtained by the 2, 4-dinitrophenylhydrazine method and the indophenol method. The values of AsA estimated by the present method and these official methods agreed well in all samples. The effect of pH and the interference of various compounds on the AsA assay were also examined.
- 社団法人 日本農芸化学会の論文
著者
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Fujita Shuji
Department Of Applied Physics Faculty Of Engineering Hokkaido University
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TONO Tetsuzo
Department of Horticultural Science, Faculty of Agriculture, Saga University
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TONO Tetsuzo
Department of Food Science and Nutrition, Nishikyushu University
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