A protease that participates in yeast cell wall lysis during zymolyase digestion.

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概要

• 論文の詳細を見る

• Zymolyase B decreased the turbidity of a yeast cell wall suspension by about 50% and caused release of peptide-mannan from the cell walls. However cell walls treated with the enzyme still maintained the cell shape. The effect of the enzyme on the cell walls was inhibited by yeast mannan and completely counteracted by treatment of the enzyme with DFP. The activity was not affected by pH, but was considerably reduced by incubation of the enzyme at 55°C for 15 min, a treatment that did not affect the proteolytic activity. Heat-treatment decreased the molecular weight of the enzyme from 29, 000 to 22, 500 and its sensitivity to yeast mannan. Yeast mannan caused noncompetitive inhibition of the proteolytic activity of the native enzyme and competitive inhibition of that of the heat-treated enzyme. Modification of tryptophan residues of Zymolyase B resulted in decreased sensitivity to yeast mannan and a decrease in the activity of the enzyme on yeast cell walls as well as heat-treatment. On the basis of these results, it is hypothesized that Zymolyase B binds to the cell wall mannans and changes their conformation, making the attached proteins susceptible to proteolysis, and then releases peptide-mannan from the cell walls.

• 公益社団法人 日本農芸化学会の論文

著者

• KITAMURA Kumpei

  The Research Laboratories of Kirin Brewery Co.

関連論文

• Lysis of Yeast Cells Showing Low Susceptibility to Zymolyase
• Isolation and identification of Crystalline Cellulose Hydrolyzing Bacterium and its Enzymatic Properties
• A High Yeast Cell Wall Lytic Enzyme-Producing Mutant of Arthrobacter luteus
• Effect of Culture Medium on Susceptibility of Yeast Cells to Zymolyase
• Re-examination of Zymolyase Purification
• A protease that participates in yeast cell wall lysis during zymolyase digestion.

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