Mass Spectrometric Determination of Cytokinins in Young Sweet-potato Plants Using Deuterium-labeled Standards

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概要

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• The presence and levels of trans- and cis-ribosylzeatin, trans- and cis-zeatin, N6-isopentenyladenosine, and 6-(3-methyl-2-butenylamino)-9-?A-D-glucopyranosylpurine were determined in the root, and the stem and leaf of young sweet-potato plants by mass spectrometry using deuterium- labeled standards. The 6-(3-methyl-2-butenylamino)-9-?A-D-glucopyranosylpurine level was found to be the highest in both the root, and the stem and leaf. trans-Ribosylzeatin was present at a high level in the root. The ratio of the trans- and cis-isomers of zeatin in the root, and in the stem and leaf were determined to be 10 and 2.4, respectively. The variation in the trans/cis ratios of both zeatin and ribosylzeatin may reflect the growth stage of the organ in the plant.

著者

• Sugiyama Tamizi

  Laboratory Of Bioorganic Chemistry Faculty Of Agriculture Meiji University

• SUYE Shin-ichiro

  Laboratory of Bio-organic Chemistry, Tokyo University of Agriculture and Technology

• SUYE Shin-ichiro

  Laboratory of Bioorganic Chemistry, Tokyo Institute of Agriculture and Technology

• HASHIZUME Takeshi

  Laboratory of Bio-organic Chemistry, Tokyo University of Agriculture and Technology

関連論文

• Changes in the Chlorophyll Contents of Leaves and in Levels of Cytokinins in Root Exudates during Ripening of Rice Cultivars Nipponbare and Akenohoshi : GROWTH AND DEVELOPMENT
• Mass Spectrometric Determination of Cytokinins in Young Sweet-potato Plants Using Deuterium-labeled Standards
• Isolation and Identification of Endogenous Cytokinins from Alfalfa (Medicago saliva L.)
• Isolation and Identification of cis-Zeatin Riboside from Tubers of Sweet Potato (Ipomoea batatas L.)

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