Isolation and Characterization of Oligosaccharides from the Hydrolyzate of Larch WoodGlucomannan with Endo-β-D-Mannanase

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概要

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• The glucomannan isolated from larch holocellulose was hydrolyzed by a purified endo-D-β-mannanase. The products were fractionated by gel filtration on a polyacrylamide gel in water and partition chromatography on ion exchange resins in 80%ethanol. The following oligosaccharides were isolated and identified: (a) 4-O-β-D-Manp-D-Man, (b) 4-O-β-D-Glcp-D-Man, (c) 4-O-β-d-Glcp-D-Glc, (d) O-β-D-Manp-(1→4)-O-β-D-Manp-(1→4)-D-Man, (e) O-β-D-Glcp(1→4)-O-β-D-Manp-(1→4)-D-Man, (f) O-β-D-Manp-(1→4)-O-β-D-Glcp-(1→4)-D-Man, (g) O-β-D-Manp-(1→4)-O-[α-D-Ganp-(1→6)]-D-Man, (h) O-β-D-Manp-(1→4)-O-β-D-Manp-(1→4)-O-β-D-Manp-(1→4)-D-Man, and (i) O-β-D-Manp-(1→4)-O-β-D-Manp-(1→4)-O-β-D-Manp-(1→4)-D-Man.

著者

• SHIMIZU Kazumasa

  Forestry and Forest Products Research Institute

• ISHIHARA MITSURO

  Forestry and Forest Products Research Institute

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