Adsorption and Desorption of Cellulase Components during Enzymatic Hydrolysis of Steamed Shirakamba (Betula platyphylla Skatchev) Wood
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概要
- 論文の詳細を見る
The adsorption behavior of Trichoderma viride cellulases on steamed shirakamba (Betula platyphylla Skatchev) wood was studied. Substrate/enzyme (S/E) ratio, hydrolysis time and saccharification extent as well as the presence or absence of lignin in the substrate were taken into consideration as factors affecting the enzyme adsorption. Filter paperase (FPase) components were adsorbed more selectively than other cellulase components on the substrate. The presence of lignin in the steamed hardwood tended to slow down the enzyme adsorption, but it did not appear to restrict the extent of hydrolysis of the carbohydrate moiety. The changes in the composition of the enzyme preparation during the course of hydrolysis were analyzed by fast protein liquid chromatography (FPLC). The irreversible adsorption of specific cellulase components was not observed in the prolonged hydrolysis of steamed shirakamba wood containing abundant lignin. The removal of lignin from steamed hardwood prior to hydrolysis is not always necessary for the recycling to cellulolytic enzymes.
- 公益社団法人日本生物工学会の論文
- 1991-08-25
著者
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SHIMIZU Kazumasa
Forestry and Forest Products Research Institute
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Hayashi Noriko
Forestry And Forest Products Res. Inst. Ibaraki Jpn
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Hayashi N
Forestry And Forest Products Research Institute
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Uemura Shoko
Forestry And Forest Products Research Institute
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ISHIHARA MITSURO
Forestry and Forest Products Research Institute
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JELLISON JODY
Department of Botany and Plant Pathology, University of Maine
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Jellison Jody
Department Of Botany And Plant Pathology University Of Maine
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Hayashi Noriko
Forestiy and Forest Products Research Institute
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