Estimation of DNA Base Composition by High Performance Liquid Chromatography of Its Nuclease PI Hydrolysate
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概要
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In order to develop a new method for determination of the G+C content of a DNA, preparations from 26 bacterial strains and salmon sperm were digested to form 5-deoxyribonucleotide-monophosphates (dNMP) with nuclease P1 and subjected to high performance liquid chromatography (HPLC). Chromatograms indicated excellent separation of the components, and the data analysis suggested sufficient reproducibility and reasonable A/T and G/C ratios over a wide range (39 to 72mol%) of G+C values.
- 社団法人 日本農芸化学会の論文
著者
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KATAYAMA-FUJIMURA Yoko
Tokyo University of Agriculture & Technology
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KOMATSU Yoko
Tokyo University of Agriculture & Technology
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KURAISHI Hiroshi
Tokyo University of Agriculture & Technology
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KANEKO Takichi
The Institute of Physical and Chemical Research
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KOMATSU Yoko
Tokyo University of Agriculture & Technology
関連論文
- Estimation of DNA Base Composition by High Performance Liquid Chromatography of Its Nuclease PI Hydrolysate
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