Cloning of Genes Encoding Oxidation of Benzene in Pseudomonas putida and Their Expression in Escherichia coli and P. putida
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概要
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A gene cluster from the chromosomal DNA of Pseudomonas putida encoding the oxidation of benzene to catechol was cloned into a broad-host-range cosmid vector, pMMB33. Escherichia coli cells containing these cloned genes were able to accumulate catechol from benzene and showed green pigmentation, presumably, due to the accumulation of indigo produced by benzene oxidizing enzymes. A mutant strain, 81CB46, of P. putida, which is deficient in the oxidation of benzene to catechol, gained the ability to grow on benzene due to the cloned genes. The functional region of the cloned fragment was less than 5 kb in length.
- 社団法人 日本農芸化学会の論文
著者
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白井 勝久
東和科学
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DOI Seiji
Central Research Laboratories, Idemitsu Kosan Co., Ltd.
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IRIE Shinji
Central Research Laboratories, Idemitsu Kosan Co., Ltd.
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SHIRAI Katsuhisa
Central Research Laboratories, Idemitsu Kosan Co., Ltd.
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YORIFUJI Toshiaki
Central Research Laboratories, Idemitsu Kosan Co., Ltd.
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