Protoplast Fusion of Lactobacillus casei
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概要
- 論文の詳細を見る
Lactobacillus casei ATCC 7469 was successfully converted to protoplasts by treatment with endo-N-acetyl muramidase in sucrose phosphate buffer. For full hydrolysis of cell walls, a high concentration of sucrose and a cold shock were necessary. Mg2+ ions enhanced the stability of protoplasting cells. The cell wall regeneration of protoplasts was more effective on gelatin-induced regeneration medium than with the soft overlay method. The optimal concentration of gelatin was 2.5%. The frequency of regeneration was found to be about 6% for the protoplast prepared by enzyme treatment for 20min. The mutants having streptomycin resistance and rifampicin resistance, as selection markers for the detection of fusion, were isolated by UV irradiation and NTG treatment. These mutants were stable for at least several transfers. Protoplast fusion was carried out using PEG (50% solution of polyethyleneglycol, M.W. 6, 000). The frequency of protoplast fusion was found to be about 10-5.
- 社団法人 日本農芸化学会の論文
著者
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Kim Jung
Department Of Applied Bioscience Cha University
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RYU Dewey
University of California
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KIM Jung
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology
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KANG Yup
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology
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