Removal of cumulus cells before oocyte nuclear maturation enhances enucleation rates without affecting the developmental competence of porcine cloned embryos

スポンサーリンク

概要

論文の詳細を見る
The present study compared the efficiency of somatic cell nuclear transfer (SCNT) usingporcine oocytes that were denuded of their cumulus cells at different maturation time. Inpre-denuded group, the cumulus cells from cumulus-oocyte complexes (COCs) wereremoved at 29 hr post in vitro maturation (hpm) and followed by further culture for 12 hr.In control group, as a commonly followed procedure, cumulus cells were removed fromCOCs at 41 hpm. The majority of porcine oocytes at 29 hpm were observed in metaphase ofthe first meiotic division (MI). At 41 hpm, no significant (P ＞ 0.05) differences wereobserved in nuclear maturation and mitochondrial distribution of oocytes betweenpre-denuded and control groups. However, in pre-denuded group oocytes, metaphase II(MII) plate and spindle were located closely as ‘adjacent’ to the first polar body (PB1),resulting in an increased enucleation rates than in control group oocytes by blindenucleation method. Following SCNT and parthenogenesis (PA) using pre-denuded groupand control group oocytes, no significant (P ＞ 0.05) differences were observed with respectto the development, total cell number, incidence of apoptosis and the expression profile ofdevelopmentally important genes (Pou5f1, Dnmt1, Dnmt3a, Igf2r, Bax, Bcl2 and Glut1) atthe blastocyst stage. In conclusion, the removal of cumulus cells at 29 hpm in porcineoocytes increased the enucleation rates through proper positioning of PB1 withoutcompromising the quality of SCNT embryos during preimplantation development. Hence,this could be a valuable strategy to improve the SCNT efficiency in a porcine model.

著者

関連論文

スポンサーリンク