Effect of Histone Acetylation Modification with Sodium Butyrate, a Histone Deacetylase Inhibitor, on Cell Cycle, Apoptosis, Ploidy and Gene Expression in Porcine Fetal Fibroblasts

スポンサーリンク

概要

• 論文の詳細を見る

• The present study evaluated the effective dose of sodium butyrate (NaB), a histone deacetylase (HDAC) inhibitor, for determination of the level of enhancement of histone acetylation in porcine fetal fibroblasts (PFFs) based on their morphology, growth, apoptosis and cell cycle status. Cells were analyzed for their histone acetylation levels at H3, H4 and H2A and expression of genes related to histone deacetylation (HDAC1, HDAC2 and HDAC3), pro-apoptosis (Bax, Bak) and anti-apoptosis (Bcl-2). PFFs at passage 3-4 were cultured with 0, 0.5, 1.0, 2.0 and 3.0 mM NaB for 96 h. NaB inhibited cell proliferation at all tested concentrations in a dose-dependent manner. However, there was slow cell growth for PFFs treated with 2.0 and 3.0 mM NaB compared with those of untreated PFFs and those treated with other lower concentrations (0.5 and 1.0 mM). More than 85% of the cells that were untreated or treated with 0.5 or 1.0 mM NaB had intact membranes, whereas, approximately 30% of the cells treated with 2.0 or 3.0 mM NaB had increased cell sizes and a more flattened and elongated appearance. NaB induced apoptosis in a dose-dependent manner; the rates of apoptosis were 2.5 ± 0.4% for 1.0 mM NaB, 7.6 ± 1.1% for 2.0 mM NaB and 11.2 ± 1.4% for 3.0 mM NaB. The chromosomal sets of PFFs treated with 0.5 and 1.0 mM NaB were normal, whereas a lower proportion of PFFs treated with 2.0 and 3.0 mM were classified as normal. NaB at 0.5 and 1.0 mM showed little effect on cell cycle. However, 2.0 and 3.0 mM resulted in an increased cell population at the G0/G1 phase. Increased NaB concentrations led to elevated acetylation of H3, H4 and H2A. NaB altered the expression of histone deacetylation and apoptosis- related genes. In conclusion, 1.0 mM NaB induced histone hyperacetylation in the PFFs and produced less deleterious effects than other concentrations; these PFFs might serve as suitable donors for porcine somatic cell nuclear transfer (SCNT).

• 日本繁殖生物学会の論文
• 2007-08-01

著者

• Cho Seong-keun

  韓国

• Rho Gyu-jin

  Res. Inst. Of Life Sciences Gyeongsang National Univ.

• Song Hye-jin

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• MOHANA KUMAR

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• CHO Seong-Keun

  Division of Applied Life Science, Gyeongsang National University

• BALASUBRAMANIAN Sivasankaran

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• CHOE Sang-Yong

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• RHO Gyu-Jin

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• Cho Seong-keun

  Division Of Applied Life Science Gyeongsang National University

• Mohana Kumar

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Choe Sang-yong

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

• Balasubramanian Sivasankaran

  Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University

関連論文

• Developmental Ability of Miniature Pig Embryos Cloned with Mesenchymal Stem Cells
• Effect of Histone Acetylation Modification with Sodium Butyrate, a Histone Deacetylase Inhibitor, on Cell Cycle, Apoptosis, Ploidy and Gene Expression in Porcine Fetal Fibroblasts
• Removal of cumulus cells before oocyte nuclear maturation enhances enucleation rates without affecting the developmental competence of porcine cloned embryos

スポンサーリンク