[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較

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The purpose of this paper is to elucidate the hydrolytic processes and the physicochemical properties of the initial hydrolysate of thymus DNA by DNase I (EC-3.1.4.5) in the presence of a very low concentration of Mg^<2+>. By examining the increasing curve on the acid soluble material and hyperchromicity, the hydrolytic process of DNA was limited by DNase I under very low Mg^<2+>concentration. The partially hydrolyzed product (ph-DNA) did not show any spectral changes by the disruption of hydrogen bonds involving the reactive amino acid. Therefore, ph-DNA is considered to still retain a double helical structure. ph-DNA was obtained from the reaction mixture under hydrolytic condition and when more Mg^<2+> was added to the reaction mixture, the b-form DNA was produced. It may be concluded that the ph-DNA is the initial degradation product of DNA obtainable by the action of DNase I in the presence of trace Mg^<2+>.
琉球大学保健学部の論文