[原著]Steady-State Characteristics of Reconstituted Glycolytic Pathway and Regulation of Flux of Pathway by Cooperation of Metabolites and Enzymes

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Glycolytic system was reconstructed with purified enzymes, inorganic ions and nucleotides for the analysis of the steady-state characteristics of the glycolytic pathway. Each amount of the enzymes used was 1.0 international unit except for the enzymes as a variable one. Amounts of the constituents of glycolysis were varied so as to obtain the control parameters such as control strength and sensitivity coefficients which characterize the dynamical properties of the pathway. GF was strongly enhanced in the presence of 0.1mM or more of AMP, and it was also enhanced by ADP and ATP until the concentration of about 2mM. Effect of ATP/ADP ratio showed most efficient effect on GF at one to one ratio. Constant level of redox state was maintained by the addition of appropriate amounts of GDH. And moreover, arbitrary steady-state of glycolysis was produced by maintaining NADH/NAD^+ ratio at constant level. Time for attaining definite GF depended direct-proportionally on NAD^+ level. PFK and GAPDH are known to effect on GF as the allosteric enzymes of the pathway. These enzymes had large control parameters showing the effective enhancement of GF. PGK had small values of parameters, but had large values with cooperation of GAPDH. On one hand GAPDH alone strongly affected GF. This may contradict the fact that these enzymes facilitate the ATP formation at substrate level to effect GF because they locate on a sequence of process of the pathway. Rapaport reported on the steady-state analysis of glycolysis in erythrocytes. The results obtained in the reconstructed system were compared with those by Rapaport and discussed.
琉球大学保健学部の論文
1980-00-00

[原著]Steady-State Characteristics of Reconstituted Glycolytic Pathway and Regulation of Flux of Pathway by Cooperation of Metabolites and Enzymes

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琉球大学保健学部 | 論文

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