[原著]手術時手洗い評価法としてのアデノシン三リン酸測定法と寒天培地法の比較

概要

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The glove juice sampling method is too complicated to use for daily evaluation in surgical hand antisepsis. While agar method is widely used to evaluate hand hygiene, the measurement of adenosine triphosphate (ATP) using bioluminescent method (ATP method) becaome easy. There is possibility of using this method to evaluate surgical hand antisepsis. The purpose of this study is to compare ATP method and agar method. The subjects are forty-seven medical students and doctors. The number of colonies before and after surgical hand antisepsis ranged 1 to 652 and 0 to 134 respectively. The reduction rate was 98.7 ± 3.2%. ATP volume before and after surgical hand antisepsis ranged 305 to 16389 RLU, and 9 to 577 RLU respectively. The reduction rate was 92.8 ± 10.6%. The correlation coefficient between the number of colonies and ATP volume was 0.79 (p<0.0001). These results suggest that ATP method is useful in evaluating surgical hand antisepsis. Comparison of ATP method and glove juice sampling method is needed.
琉球医学会,Ryukyu Medical Associationの論文