Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens

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概要

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• Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.

著者

• Shimamoto Y

  Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University

関連論文

• Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens
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