YLBSによるペプチドライブラリーの作成と利用
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概要
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Peptides have a big potential to be used for biologically active molecules such as hormone, antibiotics, inhibitors and others. Therefore, they are one of the most important substances targeted by Evolutionary Molecular Engineering. The possibility of building peptide-coding DNA libraries, beginning from 20 species of amino acid-monomer blocks, by use of YLBS technology was examined. Three/ four steps of Y-ligation were performed, providing y3 N 4 DNA libraries corresponding to peptides of 8-mers/16-mers, respectively. Y3 was confirmed to be really successful in containing the whole set of 8mers. Tenacious problems of deletion and uneven appearance of blocks were challenged and fruitfully solved by introducing an operation which does not depend on the second-strand cutting of DNA by restriction enzymes or by starting with preferential blocks for T4 RNA ligation.
- 埼玉大学地域共同研究センターの論文
埼玉大学地域共同研究センター | 論文
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