Single-cell observation of phagocytosis by human blood dendritic cells.

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概要

• 論文の詳細を見る

• Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.

• National Institute of Infectious Diseasesの論文

著者

• Ida Hiroaki

  長崎大学 医学部第2内科

• Yamanaka Kiyoharu

  北里大学 医学部微生物・寄生虫学

• Yanagihara Katsunori

  長崎大学 医学部第二内科

• Yanagihara Katsunori

  長崎大学 医歯薬学総合研究科臨床検査医学講座

• Yanagihara Katsunori

  Dep. Of Lab. Medicine Nagasaki Univ. Graduate School Of Biomedical Sciences Nagasaki Univ. Hospital

• Yanagihara Katsunori

  Second Dep. Of Internal Medicine Nagasaki Univ. School Of Medicine

• Ishii Hiroshi

  長崎大学 医学部第二内科学

• Yanagihara Katsunori

  長崎大学 第二内科

• Yanagihara Katsunori

  Department Of Laboratory Medicine Nagasaki University School Of Medicine

• Yamaguchi Kazunari

  Department Of Safety Research On Blood And Biologics National Institute Of Infectious Diseases

• ARAKI Nobuko

  Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences

• Araki Nobuko

  Department Of Laboratory Medicine Nagasaki University Graduate School Of Biomedical Sciences

• Ishii Hiroshi

  Department Of Internal Medicine Isahaya Insurance General Hospital

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