Single-cell observation of phagocytosis by human blood dendritic cells.
スポンサーリンク
概要
- 論文の詳細を見る
Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.
- National Institute of Infectious Diseasesの論文
著者
-
Ida Hiroaki
長崎大学 医学部第2内科
-
Yamanaka Kiyoharu
北里大学 医学部微生物・寄生虫学
-
Yanagihara Katsunori
長崎大学 医学部第二内科
-
Yanagihara Katsunori
長崎大学 医歯薬学総合研究科臨床検査医学講座
-
Yanagihara Katsunori
Dep. Of Lab. Medicine Nagasaki Univ. Graduate School Of Biomedical Sciences Nagasaki Univ. Hospital
-
Yanagihara Katsunori
Second Dep. Of Internal Medicine Nagasaki Univ. School Of Medicine
-
Ishii Hiroshi
長崎大学 医学部第二内科学
-
Yanagihara Katsunori
長崎大学 第二内科
-
Yanagihara Katsunori
Department Of Laboratory Medicine Nagasaki University School Of Medicine
-
Yamaguchi Kazunari
Department Of Safety Research On Blood And Biologics National Institute Of Infectious Diseases
-
ARAKI Nobuko
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences
-
Araki Nobuko
Department Of Laboratory Medicine Nagasaki University Graduate School Of Biomedical Sciences
-
Ishii Hiroshi
Department Of Internal Medicine Isahaya Insurance General Hospital
関連論文
- Low Expression of T-cell Co-stimulatory Molecules in Bone Marrow-Derived Dendritic Cells in a Mouse Model of Chronic Respiratory Infection with Pseudomonas Aeruginosa
- Genetic Diagnosis of Community-Acquired MRSA: A Multiplex Real-Time PCR Method for Staphylococcal Cassette Chromosome mec Typing and Detecting Toxin Genes
- Combined Use of Clarithromycin and Antimycobacterial Agents in Mycobacterium avium Complex Chronic Pulmonary Infection
- Lung fibrosis 10 years after cessation of bleomycin therapy.
- A Probable Case of Aspiration Pneumonia Caused by Candida glabrata in a Non-neutropenic Patient with Candidemia
- Clinical Characteristics of Pneumonia Caused by Penicillin Resistant and Sensitive Streptococcus pneumoniae in Japan
- Synergistic effect of ofloxacin and fluconazole against azole-resistant Candida albicans
- Antifungal activity of a new triazole, voriconazole(UK-109496), against clinical isolates of Aspergillus spp
- Giant Cell Interstitial Pneumonia in a 15-year-old Boy
- Bronchiolitis Obliterans Organizing Pneumonia Induced by Minocycline
- Juvenile Idiopathic Nonspecific Interstitial Pneumonia. and Review of Literature
- Respiratory Bronchiolitis-associated Interstitial Lung Disease
- A Rare Case of Eosinophilic Pleuritis due to Sparganosis
- Single-cell observation of phagocytosis by human blood dendritic cells.
- High-resolution melting analysis for a reliable and two-step scanning of mutations in the tyrosine kinase domain of the chimerical bcr-abl gene.
- Elevation of Serum KL-6 Glycoprotein or Surfactant Protein-D in Adult T-cell Leukemia with Distinct Pulmonary Complications
- A Patient with Fulminant Primary Influenza Pneumonia Which Developed into Secondary Bacterial Pneumonia
- Quorum Sensing System Lactones Do Not Increase Invasiveness of a MexAB-OprM Efflux Mutant but Do Play a Partial Role in Pseudomonas aeruginosa Invasion
- Clinical Comparative Study of Sulbactam/Ampicillin and Imipenem/Cilastatin in Elderly Patients with Community-Acquired Pneumonia
- Single-cell-based-observation of macrophage's phagocytic responses and the alteration of its morphology by activation
- A Solitary Bronchial Papilloma with Malignant Changes
- Genetic Diagnosis of Community-Acquired MRSA: A Multiplex Real-Time PCR Method for Staphylococcal Cassette Chromosome mec Typing and Detecting Toxin Genes
- Low Expression of T-cell Co-stimulatory Molecules in Bone Marrow-Derived Dendritic Cells in a Mouse Model of Chronic Respiratory Infection with Pseudomonas Aeruginosa
- Disease Severity in Patients with Simultaneous Influenza and Bacterial Pneumonia
- Linezolid-resistant Staphylococcus aureus isolated from 2006 through 2008 at six hospitals in Japan
- Criteria for safety evaluation of antimicrobial agents
- Intra- and inter-laboratory variability in human T-cell leukemia virus type-1 proviral load quantification using real-time polymerase chain reaction assays : A multi-center study
- Clinical Features of Bacteremia Caused by Methicillin-Resistant Staphylococcus aureus in a Tertiary Hospital
- Nationwide surveillance of bacterial respiratory pathogens conducted by the Japanese Society of Chemotherapy in 2008 : general view of the pathogens' antibacterial susceptibility
- In vivo efficacy of doripenem (DRPM) against Pseudomonas aeruginosa in murine chronic respiratory tract infection model.
- Clinical Characteristics of Seven Patients with Aeromonas Septicemia in a Japanese Hospital
- Characteristics and disease severity of healthcare-associated pneumonia among patients in a hospital in Kitakyushu, Japan
- Active surveillance of methicillin-resistant Staphylococcus aureus with the BD GeneOhm MRSA™ assay in a respiratory ward in Nagasaki, Japan
- Pulmonary Cryptococcosis Presenting as Endobronchial Lesions in a Patient under Corticosteroid Treatment
- An Instructive Case Suggesting Warfarin Resistance Which is Independent on the Regulation of the CYP2C9 and VKORC1 Genotype
- The efficacy and safety of high-dose arbekacin sulfate therapy (once-daily treatment) in patients with MRSA infection
- Heterogeneity in clonal nature in the smoldering subtype of adult T-cell leukemia : continuity from carrier status to smoldering ATL
- Clinical Characteristics of Tertiary Hospital Patients from Whom Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Strains were Isolated
- In vivo efficacy and pharmacokinetics of biapenem in a murine model of ventilator-associated pneumonia with Pseudomonas aeruginosa
- Leukocytoclastic Vasculitis after Pneumococcal pneumonia in an Elderly Adult
- Necrotizing Pneumonia due to Femoral Osteomyelitis Caused by Community-acquired Methicillin-resistant Staphylococcus aureus
- The definition of healthcare-associated pneumonia (HCAP) is insufficient for the medical environment in Japan : a comparison of HCAP and nursing and healthcare-associated pneumonia (NHCAP)
- Detection of Legionella pneumophila serogroup 1 in blood cultures from a patient treated with tumor necrosis factor-alpha inhibitor
- Molecular characterization of quinolone-insensitive Streptococcus pneumoniae isolates from Japanese patients