光電管溷濁計を用いた抗原抗体反応の研究

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We can draw some conclusions as to the form of antigen antibody reaction from turbidimetorical examinations of precipitin reaction between ovalbumin and antiovalbumin, and diphtheria toxoid and antitoxoid. The nephelometer used in these experiments is constructed by authors. 1. Flocculation of antigen-antibody complexes obeys the theoretical relationships in colloidal solutions. If the concentration of antigen-antibody decreases in 1/2, the velocity of turbidity curve diminishes in 1/4. Generally the velocity of flocculation is proportional to amounts of reagents. This relationship agreed perfectly with the curves in optimal ratio, excess of antigen and excess of antibody zone. 2. The series of two fold diluted antigens are added to the constant antibodies; in this case the standard curve is optimal ratio, in antigen excess zone 6 curves are observed, and the height of these curves diminished in 1/1.6 proportional to 2 fold dilution of antigen, while in antibody excess zone 1 at most 2 curves are observed. On this basis, antibody seems usually monovalent, or at most divalant. 3. When flocculation times in minutes of different mixture of diphtheria toxoid and antitoxoid are calculated by the nephelometer, a series of determinations of a constant antibody or of a constant antigen gives a system of data, and when this data are plotted inversaly in three dimensions, we recognize a surface which shows the position of optimal ratio as the top line of concurved surface. Two dimensional graphs can be made which are really just appropriate sections of this.
千葉医学会の論文
1952-11-28

著者

神山一郎
千葉大学医学部衛生学教室

光電管溷濁計を用いた抗原抗体反応の研究

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