Intracellular Trafficking Pathway of Yeast Long-chain Base Kinase Lcb4, from Its Synthesis to Its Degradation
スポンサーリンク
概要
- 論文の詳細を見る
Sphingoid long-chain base 1-phosphates act as bioactive lipid molecules in eukaryotic cells. In budding yeast, long-chain base 1-phosphates are synthesized mainly by the long-chain base kinase Lcb4. We recently reported that, soon after yeast cells enter into the stationary phase, Lcb4 is rapidly degraded by being delivered to the vacuole in a palmitoylation- and phosphorylation-dependent manner. In this study, we investigated the complete trafficking pathway of Lcb4, from its synthesis to its degradation. After membrane anchoring by palmitoylation at the Golgi apparatus, Lcb4 is delivered to the plasma membrane (PM) through the late Sec pathway and then to the endoplasmic reticulum (ER). The yeast ER consists of a cortical network juxtaposed to the PM (cortical ER) with tubular connections to the nuclear envelope (nuclear ER). Remarkably, the localization of Lcb4 is restricted to the cortical ER. As the cells reach the stationary phase, G1 cell cycle arrest initiates Lcb4 degradation and its delivery to the vacuole via the Golgi apparatus. The protein transport pathway from the PM to the ER found in this study has not been previously reported. We speculate that this novel pathway is mediated by the PM-ER contact.
- American Society for Biochemistry and Molecular Biologyの論文
- 2007-09-28
著者
-
Igarashi Yasuyuki
Department Of Biomembrane And Biofuntional Chemistry Graduate School Of Pharmaceutical Sciences Hokk
-
Sassa Takayuki
Hokkaido Univ. Sapporo Jpn
-
Iwaki Soichiro
Nagoya City Univ. Nagoya Jpn
関連論文
- Sphingosine 1-Phosphate Breakdown in Platelets
- Sphingosine 1-Phosphate Formation and Intracellular Ca^ Mobilization in Human Platelets: Evaluation with Sphingosine Kinase Inhibitors
- Quantification of Sphingosine Derivatives in Human Platelets: Inducible Formation of Free Sphingosine^1
- Sphingosine 1-Phosphate, a Bioactive Sphingolipid Abundantly Stored in Platelets, Is a Normal Constrituent of Human Plasma and Serum^1
- Lack of sphingosine 1-phosphate-degrading enzymes in erythrocytes
- GM3 synthase gene is a novel biomarker for histological classification and drug sensitivity against epidermal growth factor receptor tyrosine kinase inhibitors in non-small cell lung cancer
- Development of a New Inhibitor of Glucosylceramide Synthase^1
- Palmitoylation of the sphingosine 1-phosphate receptor S1P1 is involved in its signaling functions and internalization
- Intracellular Trafficking Pathway of Yeast Long-chain Base Kinase Lcb4, from Its Synthesis to Its Degradation
- The immunomodulator FTY720 is phosphorylated and released from platelets
- Regulation of the transport and protein levels of the inositol phosphorylceramide mannosyltransferases Csg1 and Csh1 by the Ca2+ binding protein Csg2
- Changes in S1P(1) and SIP(2) expression during embryonal development and primitive endoderm differentiation of F9 cells
- Intracellular localization and tissue-specific distribution of human and yeast DHHC cysteine-rich domain-containing proteins
- ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis