日本脳炎ウイルスの血球凝集反応に及ぼすTween 80の影響
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概要
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Daiflon 113, a fluorocarbon, was available for extraction of Japanese encephalitis (JE) virus antigen from infected mouse brain. The fluorocarbon extract was not only effective as antigen for hemagglutination and complement fixation, but reservable efficiently at least for three months. HA activity of Nakayama NIH antigen prepared by the fluorocarbon method was promoted by adding Tween 80 with the final concentration of 0.5 percent, while that of JaGAr 01 antigen was markedly depressed. On the other hand, HA of aceton-ether antigens was inhibited by Tween 80 to a great extent in both the strains. Thirteen out of 17 wild strains which had been isolated from mosquitoes in the epidemic season of JE, showed the depression of HA due to Tween 80 as was observed in JaGAr 01 strain, but the remaining four were little influenced. Although no significant reduction of CF activity could be observed in both the strains, it was found that the infectivity of the strains to a cultured cell line had been markedly reduced. Using antigens prepared by fluorocarbon or aceton-ether method, cross HI tests were carried out. The antigens of JaGAr 01 strain showed approximately the same HI as to homologous and heterologous antisera, but in case of Nakayama strain some reduction in titer against heterologous antisera was observed. It is conceivably possible to consider that discrepancies between the findings of the strains lies in different character of their hemagglutinins.
- 長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文
- 1970-03-20
長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki University | 論文
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