宮入貝卵子の発育に及ぼす塩類の影響
スポンサーリンク
概要
- 論文の詳細を見る
産卵直後の宮入貝卵子を用い,その卵子を被っている泥皮を人工的に摘除した後の裸の卵子を25℃の恒温のもとに種々の塩類溶液で培養を行ない,次の結果を得た.1)卵子は蒸留水や単なる NaCl だけの溶液ではいかなる濃度でもなかの胚子は発育しない.2) NaCl 2.9g, KCl 0.05g, CaCl_2・2H_2O 0.13g,MgSO_4・7H_2O 0.05g, NaHCO_3 0.20g/l の夫々の量を含む溶液(標準液)の中で培養を行なうと胚子は順調に発育して, 16日前後に卵膜を破って孵化する。3) pH 7.1～8.3の範囲内で胚子の発育は順調である.4) NaClの濃度では3.1g/l(浸透圧が111 mOsm)以下の量であれば,たとえ NaClを欠いても他の成分があれば胚子は発育できる.5) KCl, CaCl_2, MgSO_4, NaHCO_3の濃度では,その量が夫々KClで0.04～0.20g/l, CaCl_2・2H_2Oで0.11～0.50g/l, MgSO_4・7H_2O で0.05～0.11g/l,NaHCO_3で0.16～0.40g/lの範囲内にある溶液に於て,胚子の発育は良好である.The authors have been making a series of laboratory investigations to find the most ideal condition for survival and development of Oncomelania eggs. As the second step of the study, the authors studied the effect of various mineral salts on the fresh eggs of Oncomelania nosophora which were taken off their mud-like covering. In simple NaCl solution as distilled water the naked eggs did not develop or died in a few days showing degeneration, regardless of the concentration of NaCl in the solution. Therefore, as a standard culture solution we used the mixed salts solution which contains NaCl 2.9g, KCl 0.05g, CaCl_2・2H_2O 0.13g, MgSO_4・7H_2O 0.05g and NaHCO_3 0.20g per liter, showing pH 8.1 and 105 mOsm of osmolarity. In this standard solution kept at 25℃, the eggs normally developed to mature larvae and most of them hatched within 16 days after incubation. Higher pH over 10.2 or lower than 5.0 resulted a significant damage on the viability of the egg. The optimal potential of hydrogen of the solution seemed to be around 7.1 to 8.3, and the osmolarity must be limited under 108 mOsm. Effect of the mineral salts was tested by changing individually the amount of each component of the standard culture solution, and the following evidences were obtained: The eggs can continue their development up to maturity and hatch even in the modified standard solution lacking NaCl. On the other hand, the presence of proper amounts of KCl, CaCl_2, MgSO_4 and NaHCO_3 is indispensable for the eggs to attain maturity and hatch. The optimal amounts of respective salts are KCl 0.04 to 0.20g, CaCl_2 2H_2O・0.11 to 0.50g, MgSO_4 7H_2O 0.05 to 0.11g and NaHCO_3 0.16 to 0.40g.
- 長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文
長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki University | 論文
- Brugia pahangi感染ラット,ジャード,マウスにおける好酸球応答の差異
- フィラリアおよび糞線虫症と成人T細胞白血病ウイルス感染 : 旧フィラリア流行地・熊本県南部の過疎地における調査
- フィラリアおよび糞線虫症と成人T細胞白血病ウイルス感染 : 旧フィラリア流行地・現在の糞線虫流行地,鹿児島県喜界島における調査
- 虫垂より分離した緑膿菌のピオシン型別と血清型別
- 長崎県の2島におけるトキソプラスマ感染症に関する血清疫学的研究