プラジカンテルによるSchistosoma mansoniの虫卵の孵化に関する研究

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概要

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• Praziquantel-induced hatching of Schistosoma mansoni eggs was studied. In phosphate buffered saline (PBS) with osmolarity of 365 mOsM or 465 mOsM, most miracidia enclosed within the egg shell were immotile. Once praziquantel (PZQ) was added to PBS to give a final concentration of 1-100 μg/ml, miracidia began to take a screwlike or rotary motion within the shell. And then the egg shell was ruptured and miracidium was released. Thirty-six percent of the mature eggs hatched in PBS (465 mOsM) cotaining PZQ at a concentration of 100 μg/ml during the observation period of 2 hours at 25℃. The corresponding hatching rate of eggs in PBS without PZQ was 8.3%. PZQ-induced hatching was partially inhibited by EGTA (5mM), Lanthanum chloride (0.1-0.5mM) and Ruthenium Red (0.5-1mM). Calcium ionophore (A23187) stimulated the miracidia to swim within the shell and to hatch. Calcium ionophore, at a concentration of 10^<-4> M, allowed 20% of mature eggs to hatch in PBS (365 mOsM) during the observation period of 2 hours. When the eggs were incubated in high concentration of CaCl_2 solution (112mM), the miracidia became motile in some eggs, but they did not hatch. The break of the shell was most frequently located on the long axis of the shell opposite the spine. The edges of the broken shell were sharp, uniform in thickness, and complementary throughout the orifice. The proteinase inhibitors failed to inhibit PZQ-induced hatching. The possible role of Ca^<++> in PZQ-induced hatching process of S. mansoni eggs was disussed.

• 長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文
• 1988-03-31

著者

• 勝又 達哉

  長崎大・熱研・寄生行動制御

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• プラジカンテルによるSchistosoma mansoniの虫卵の孵化に関する研究

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