Specific Reaction of a Protein of Jack Bean with Sera of Various Animals

概要

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Jack bean contained a protein, which reacted specifically with serum proteins. This specific protein was named provisionally Protein J. Specific precipitation of serum proteins with Protein J was confirmed by simply mixing them in test tube or by the technique of agar gel diffusion. By the two-dimensional crossing paper electrophoresis, crossing diagrams of the extract of jack bean against sera on the hand, and those of sera against the extract of jack bean on the other hand, were obtained. The foemer diagrams showed a peak in the region of Protein J, indicating that the reactant was Protein J; the the latter diagrams showed usually four peaks corresponding to α1-,α2-,β-,and γ-globulin of serum. The crossing diagrams of sera of various animals showed characteristic figures. Some other beans than jack bean contained also a specific protein, which reacted in the same manner. Acknowledgement. Thanks are due to Prof. S. Nakamura for his kind guide and to Dr. K. Tanaka for his kind advice.
Yamaguchi University Graduate School of Medicineの論文