On the Separation and Identification of Sulfonamides by the Thin-Layer Chromatography and the Detection of Sulfa Drug in the Cadaveric Blood

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The autors have described the separation and identification of seven kinds of sulfonamides by means of the thin-layer chromatograghy and an example for the detection of sulfamonomethoxine sodium in the cadaveric blood. In the separation of these sulfonamides, the developers of chloroform-methanol system which consist of two or more kinds of polar and non-polar solvents, gave the satisfactory result. All these sulfonamides could be detected even in a very small quantities of 0.1 μg by using p-dimethylaminobenzaldehyde or diazo reagent and the color was very clear. As a result, both reagents would be expected to be available for the detecting reagent in the usual analysis of sulfonamides. From the blood (8 ml) of a man aged 52 who ”Daimeton” injection was intravenously received and suddenly died, sulfamonomethoxine sodium was separated and identified through the analysis coupled with the thin-layer chromatography and ultraviolet absorption spectrophotometry. The quantities of sulfamonomethoxine sodium determined about the remainder of blood extract after the qualititive analysis was approximately 3.0 mg.
Yamaguchi University Graduate School of Medicineの論文