Expression of mDNAs Encoding Adenylate Kinase Isozymes 1,2,3, and 4 in Mouse tissues and during Neuronal Differentiation of P19 Embryonal Carcinoma Cells

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We cloned cDNAs econoding four adenylate kinase (AK) isozymes from mouse kindney cDNA library. The AK1, AK2, AK3, and AK4cDNAs encode the 194-, 232-, 227-, and 223-amino acid protains, respectively. AK4 is a recently isolated gane that is highly homologous to the reported human AK3. Northern blot analysis and reverse transcriptionpolymerase chain reaction analysis revealed that AK1 mDNA was predominantly expressed in skeletal muscle, heart, and testis; AK2 mDNA in liver, hrart, kidney and testis; AK3 mDNA almost uniformly in all tissues examined; and AK4 mDNA prominently in kidney. Subcellular and submitochondrial fractination analysis suggested that AK4 was localized in the mitochondrial matrix. Further, we found a 76-fold induction of AK1 mDNA expression concomitant with a 53-fold induction of NeuroD expression during retinoic acid-induced neuronal differentiation of P19 embryonic carcinoma cell. AK2 and AK3 mDNA expression was increased by 4- to 6-fold during differentiation, whereas AK4 transcription was first down-regulated and subsequently returned to the original level. Our date on Akisozyme gane expression may provide basic information for production and evaluation of transgenic mice as well as knockout mice to further understand the physiological rote of AK isozymes.
Yamaguchi University Graduate School of Medicineの論文