Inducible Particles of Bacillus aneurinolyties．

概要

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To detect prophages and bacteriocins, twenty strains of Bacillus aneurinolyticus were treated with mitomycin C. The result ed lysates were subjected to electron microscopy, and also examined for killing and plaque-forming activities. Seventeen strains showed killing activity on two or more strains of Bacillus aneurinolyticus. Killing agents were centrifuged in linear 5 to 20% sucrose gradient, and studied with electron microscopy which revealed the presence of particles. They looked morphologically like phage tail of 190 nm long with fiber (KA10,KA17) or without fiber (KA1,KA6), T even phage-like particle with a head of 50 nm in diameter and a tail of 140 nm long (KA23), or T7 phage-like partocle with a head of 70 nm in diameter and a tail of 20 nm long (KAS232). The killing agent of KAS232 showed plaque-forming activity on several strains different from killing sensititive strains of Bacillus aneurinolyticus.
Yamaguchi University Graduate School of Medicineの論文