A Look－up Table for Coefficient of Variarion in DNA Flow Cytometry

概要

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A look-up table for estimation of the coefficient of variation (CV) during flow cytometry of cellular DNA content distribution was made by a computer simulation of the ahape of the G1 Fluorescence peak. The basic nation is that the accuracy of measurement is indicated by by the sharpness of the G1 fluorescence peak, and in contrast to the well-known FWHM method (full width of the peak at half maximal ordinate value method), it utilizes the height of three channels at the G1 peak; channels which are least affected by the presece of early S cells or cell debris. The table is two-dimensional and requres entry of the channel number of the G1 peak and of a bluntness index which is defined as the ratio of mean height of two adjacent channels to the height of the peak.
Yamaguchi University Graduate School of Medicineの論文
1984-12-00