The Analysis of GP63 on Leishmania Amastigotes and Promastigotes using Immunofluorescence Method
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Leishmania life cycle consists of two stages; the stage of extracelluar promastigotes which in the gut of sandfly insect vector and stage of intracellular amastigotes which divide in the phagolysosome of mononuclear phagocytic cells of the mammalian host. Promastigotes express a major surface glycoproteinof 63 kDa, refrred to as GP63. The expression of GP63 in both Leishmania life stages was studied using rabbit antibodies against native GP63 as well as rabbit antibodies against recombiant GP63 that was synthesized in an Escherichia coil expression system. It was demonstrated using immunofluorescence staining that GP63 antigen was not only on the surface of Leishmania promastigotes but also on that of amastigotes which were within a macrophage with anti-recombinant GP63 antibody was less intense than that with anti-GP63. The difference of GP63 antigen distribution on the surface of Leishmania mexicana and major was also shown in this paper.
- Yamaguchi University Graduate School of Medicineの論文
Yamaguchi University Graduate School of Medicine | 論文
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