Simple and sensitive method for quantification of fludarabine triphosphate intracellular concentration in leukemic cells
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概要
- 論文の詳細を見る
A simple, isocratic HPLC method was newly developed for quantitating intracellular fludarabine triphosphate (F-ara-ATP). Samples (500 µl) were injected onto an anion-exchange column and eluted isocratically with phosphate-acetonitrile buffer (flow rate: 0.7 ml/min) at an ambient temperature. F-ara-ATP was quantitated according to its peak area at the absorbance of 261 nm. The standard curve was linear with minimal within-day and inter-day variability. The low and high quantification limits were 50 pmol and 20 nmol, respectively. The method was capable of measuring F-ara-ATP generated in cultured leukemic cells in vitro. Thus, our method will be useful because of its sensitivity and simplicity as well as applicability to biological materials.
- Elsevierの論文
- 2004-01-05
著者
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Ueda Takanori
First Department Of Internal Medicine Faculty Of Medical Sciences University Of Fukui
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Ueda Takanori
First Department Of Internal Medicine Fukui Medical University
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Yamauchi Takahiro
First Department Of Internal Medicine Fukui Medical University
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