Hybrid sponge comprised of galactosylated chitosan and hyaluronic acid mediates the co-culture of hepatocytes and endothelial cells(CELL AND TISSUE ENGINEERING)

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概要

• 論文の詳細を見る

• When constructing an in vitro model of liver tissue to mimic the in vivo liver microenvironment, the major challenge is to preserve and maintain the hepatocyte phenotype. The aim of this study was to develop a novel intelligent hybrid sponge for use in a dense co-culture system designed to simulate the liver microenvironment. We prepared a galactosylated chitosan (GCs)/hyaluronic acid (HA) hybrid sponge using a freeze-drying technique for the co-culture of primary hepatocytes and endothelial cells. Subsequently, we investigated the biocompatibility of the GCs/HA scaffold with primary hepatocytes and endothelial cells in terms of cell attachment, morphology, bioactivity, and maintenance of specific liver functions. The GCs/HA-hybrid sponge demonstrated good biocompatibility not only with primary hepatocytes, but also with endothelial cells. In our model, primary hepatocytes exhibited superior bioactivity and higher levels of liver-specific functions in terms of hepatocyte-specific gene expression, urea production, and testosterone metabolism as compared to a monoculture system. We succeeded in constructing a liver tissue-like model using the GCs/HA-hybrid sponge. Therefore, we anticipate that GCs/HA-hybrid sponges may be a promising matrix for the co-culture of hepatocytes and endothelial cells in liver tissue engineering, and might be employed as a novel co-culture model for applications in toxicology and drug metabolism.

• 公益社団法人日本生物工学会の論文

著者

• Tagawa Yoh-ichi

  Department Of Biomolecular Engineering Graduate School Of Bioscience And Biotechnology Tokyo Institu

• Tamai Miho

  Department Of Biomolecular Engineering Graduate School Of Bioscience And Biotechnology Tokyo Institu

• Yang Jun

  The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University

• Nagaoka Noriyuki

  Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• Shang Yi

  Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

• Ishii Ryusei

  Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

• Yoshida Yasuhiro

  Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• Ogasawara Masamichi

  Catalysis Research Center and Graduate School of Life Science, Hokkaido University

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• Liver Organogenesis from Murine Embryonic Stem Cells(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
• Mitochondrial development of the in vitro hepatic organogenesis model with simultaneous cardiac mesoderm differentiation from murine induced pluripotent stem cells(CELL AND TISSUE ENGINEERING)
• Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis
• Hybrid sponge comprised of galactosylated chitosan and hyaluronic acid mediates the co-culture of hepatocytes and endothelial cells(CELL AND TISSUE ENGINEERING)
• Innate immunity in an in vitro murine blastocyst model using embryonic and trophoblast stem cells(CELL AND TISSUE ENGINEERING)

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