A11 血管内皮細胞における超音波によるヘムオキシゲナーゼ1遺伝子発現変化(口頭発表)

概要

論文の詳細を見る
We investigated enhancement of heme oxygenase 1 (HO1) gene expression in endothelial cells in response to sonication stimulation. When exposing endothelial cells to sonication with 1MHz ultrasound at 0.3W/cm^2 for 10 to 30sec, enhancement of HO1 expression was the most prominent at either transcription level or translation level. To explore the mechanism, we conducted promoter analysis using a series of deletion mutants, suggesting the E1 region that is located at around 4kb upstream of the HO1 coding region would be involved. In addition, when a mutation was introduced into one of the three copies of StRE that is related to oxidative stress responses in the E1 region, the promoter activation response to sonication was lost, suggesting involvement of a transcription factor capable of binding to StRE. Comprehensive analysis with microarray predicted activation of several signal transduction pathways including ones related to modulation of vascular tones in addition to one related to enhancement of HO1 expression. We will be pursuing possibility of controlling vascular tones with sonication through controlling HO1 expression.
日本ソノケミストリー学会の論文
2013-10-25