P20 超音波照射によるHSPの誘導

概要

論文の詳細を見る
Heat shock protein (HSP) induced in cells has a cytoprotective effect. Recently, the application of heat shock and medical agents to cells has been studied as one of the methods to induce HSP. However, these methods have drawbacks such as heavy burden for human body and difficulty in controlling the prescription condition. The present study focused on the chemical effects (the generation of active oxygen species) of ultrasound exposure on the induction of HSP. Our objective was to induce HSP in HuH7 cells by ultrasound exposure in vitro. The ultrasound exposure condition was at the frequency of 1MHz and the power output of 0.5〜2.3 W. To confirm the basic performance of this device, we exposed ion-exchange water by ultrasound and measured the concentration of the generated H_2O_2 by KI method. H_2O_2 was generated at power outputs over 1.4 W. HuH7 cells were exposed by ultrasound for 1 to 20 min at the intensity of 1.4 W or 2.3 W. When the device intensity was 1.4 W, HO-1 and HSP72 were not induced. However, when the exposure intensity was increased to 2.3 W, HO-1 and HSP 72 were successfully induced after 10 min and 20min of irradiation, respectively. Thus, we demonstrated that ultrasound exposure could induce HO-1 and HSP 72 in HuH7 cells..
日本ソノケミストリー学会の論文
2009-10-23