P-15 超音波による遺伝子導入におけるキャビテーションの修飾効果

概要

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The effects of acoustic cavitation on in vitro gene transfer by ultrasound were investigated. HeLa cells were exposed to 1 MHz continuous waves in culture medium containing the luciferase gene. Transfection efficiency was elevated when an echo contrast agent, Levovist was added or air was dissolved in the medium. When cells were sonicated in medium saturated with Ar, N_2, or N_2O which have different γ values (Cp/Cv), or were saturated with He, Ar, or Ne with different thermal conductivties, the effectiveness for the dissolved gases in the ultrasound mediated gene transfer was Ar > N_2 > N_2O or Ar > Ne > He, respectively. When free radical formation in water by ultrasound was monitored as a measure of inertial cavitation, it was similarly affected by dissolved gases. In addition, effects of hyperthermia and a local anesthetics, lidocaine, which increase the plasma membrane fluidity, on ultrasound mediated gene transfer in PC-3 cells were studied. Although both enhanced ultrasound mediated gene transfer, the enhancement of inertial cavitation was observed only under hyperthermic conditions. These results indicate that the efficiency of ultrasound mediated gene transfer was significantly affected either by occurrence of or by modification of inertial cavitation due to various gasses. These results also suggest that the membrane fluidity plays an important role in ultrasound mediated gene transfer.
日本ソノケミストリー学会の論文