27. 超音波を用いた酵母の殺菌

概要

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Recently, Cryptosporidium parvum, which causes a waterbome disease, has been detected in drinking water. Their destruction is the serious problem in drinking water processes for public health. Cryptosporidium parvum has high resistance to germicides because it has very hard oocyst. Therefore its inactivation by the chlorination using chlorine and chloamine is not effective. The possibility of application of the ultrasound as a physicochemical method for disinfection is expected, due to short-time high temperatures, high pressures and reactive free radicals induced by ultrasonic irradiation in water. We investigate the inactivation by ultrasonic irradiation using yeast cell (Saccharomyces cerevisiae) having a similar structure with Cryptosporidium Parvum as test species. The ultrasonic irradiation for the inactivation of yeast cell is carried out horn type of equipment (27.5kHz). After ultrasonic irradiation, the viability was observed by colony count. Yeast cell had almost completely inactivated with 3 min of ultrasonic irradiation with 4μ m(p-p) of amplitude. In this study, we describe the effect of cell concentration and amplitude of vibration face of yeast cell during ultrasonic irradiation.
日本ソノケミストリー学会の論文
2001-11-28