Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• A bifunctional xylosidase/arabinofuranosidase gene (PcXyl) was cloned from the cDNA library of Phanerochaete chrysosporium and further expressed in Pichia pastoris. Enzymatic assay indicated that P. pastoris produced rPcXyl at a level of 26,141 U 1^<-1>. The xylosidase and arabinofuranosidase activities of rPcXyl were maximized, respectively, at pHs of 5.0 and 5.5 and temperatures of 45℃ and 50℃. SDS-PAGE revealed a single band of purified rPcXyl of 83 kDa. Cu^<2+> and Zn^<2+> completely inhibited the enzyme activity of rPcXyl. The enzyme activity of rPcXyl was increased 151%, 126% and 123%, respectively, in the presence of glucose, xylose and arabinose at concentrations of 5 mM. rPcXyl hydrolyzed xylobiose to xylose and xylotriose to xylose and xylobiose, indicating rPcXyl acts as an exo-type enzyme. Additionally, rPcXyl enhanced xylose release from xylan substrates in synergy with rPcXynC.

• 公益社団法人日本生物工学会の論文

著者

• Huy Nguyen

  Division Of Biotechnology College Of Environmental And Bioresource Sciences Chonbuk National Univers

• Park Seung-moon

  Division Of Biotechnology College Of Environmental & Bioresource Sciences Chonbuk National University

• Park Seung-Moon

  Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University

• Huy Nguyen

  Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University:Institute of Resources, Environment and Biotechnology, Hue University

• Thayumanavan Palvannan

  Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University

• Kwon Tae-Ho

  Natural Bio-Materials Co.

関連論文

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• Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochaete chrysosporium(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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