Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochaete chrysosporium(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• The cDNA of acetyl xylan esterase 2 (PcAxe2) gene containing a carbohydrate binding module (CBM) sequence from Phanerochaete chrysosporium was cloned and expressed in Pichia pastoris. The recombinant PcAxe2 protein (rPcAxe2) was efficiently produced, reaching a maximum of 1058 U1^<-1> after 6 days of cultivation. Molecular mass of the rPcAxe2 on SDS-PAGE was approximately 63 kDa under hyperglycosylation. Optimal activity of the purified rPcAxe2 enzyme was observed at pH and temperature of 7.0 and 30-35℃, respectively. In addition to acetyl xylan esterase activity, rPcAxe2 also exhibited a xylanase activity at an optimum pH and temperature of 5.0 and 80℃, respectively. The synergistic action of rPcAxe2 with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan enhanced the total reducing soluble sugar.

• 公益社団法人日本生物工学会の論文
• 2013-05-00

著者

• Huy Nguyen

  Division Of Biotechnology College Of Environmental And Bioresource Sciences Chonbuk National Univers

• Park Seung-moon

  Division Of Biotechnology College Of Environmental & Bioresource Sciences Chonbuk National University

• Park Seung-Moon

  Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University:LED-Agribio Fusion Technology Research Center, Chonbuk National University

• Thiyagarajan Saravanakumar

  Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University

• Kim Dae-Hyuk

  Department of Molecular Biology, College of Natural Sciences, Chonbuk National University

• Huy Nguyen

  Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University:Institute of Resources, Environment and Biotechnology, Hue University

関連論文

• Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Acetyl xylan esterase of Aspergillus ficcum catalyzed the synthesis of peracetic acid from ethyl acetate and hydrogen peroxide(ENVIRONMENTAL BIOTECHNOLOGY)
• Xylarinols A and B, two new 2-benzoxepin derivatives from the fruiting bodies of Xylaria polymorpha
• Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochaete chrysosporium(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

スポンサーリンク