Cloning, expression and biochemical characterization of a novel, moderately thermostable GDSL family esterase from Geobacillus thermodenitrificans T2(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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A thermostable GDSL family esterase-encoding gene, EstL5, was directly obtained from the genomic DNA of Geobacillus thermodenitrificans T2. Recombinant hexahistidine-tagged EstL5 was overexpressed, purified, and its biochemical properties were partially characterized. EstL5 was observed to be active within the temperature range of 0-80℃, having maximal activity at 60℃. Unlike most other thermostable enzymes, EstL5 displayed 24% of its highest activity at 0℃. EstL5 exhibited a high level of activity within a pH range of 6.0-11.0, showing the highest activity at pH 8.0. EstL5 also retained 100% of its activity after a 12-h incubation at 55℃. Furthermore, this enzyme was observed to be strongly inhibited by 10% (w/v) SDS and 0.1 mM PMSF.
- 2013-02-00
著者
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Xie Yi
State Key Lab. Of Genetic Engineering Inst. Of Genetics School Of Life Sciences Fudan Univ.
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Mao Yumin
State Key Laboratory Of Genetic Engineering Institute Of Genetics School Of Life Science Fudan Unive
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Ji Chaoneng
State Key Laboratory Of Genetic Engineering Institute Of Genetics School Of Life Sciences Fudan Univ
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Yang Zhenxing
State Key Laboratory Of Genetic Engineering Institute Of Genetics School Of Life Sciences Fudan Univ
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Zhang Yong
State Key Laboratory For Advanced Metals And Materials University Of Science And Technology Beijing
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XIE Yi
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University
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Zhang Yong
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University
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Ji Chaoneng
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University
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Shen Tiantian
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University
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Mao Yumin
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University
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- Cloning, expression and biochemical characterization of a novel, moderately thermostable GDSL family esterase from Geobacillus thermodenitrificans T2(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)