A-6 水分散性ナノメタル粒子のマクロファージに対する影響(組織・細胞,一般講演(口頭発表),第60回日本歯科理工学会学術講演会(創立30周年記念大会))

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This study evaluated biological responses of water-dispersible gold (Au-NPs) and silver nanoparticles (Ag-NPs) stabilized by metal-C σ-bonds in cultured murine macrophages (RAW264.7) using assay of cell viability, LDH release, RT-qPCR, and morphological analyses. These results suggested that the Ag-NPs showed cytotoxicity that depended on the NP concentration. In contrast, no cytotoxic effects were observed in the Au-NPs. The expression level of IL-1α was increased after Au- and Ag-NP exposure. The NPs were taken up with minor cell morphological changes for Au-NPs compared to Ag-NPs, in which particles aggregated in the vesicles. The cell endocytosis may induce harmful effects due to expansion of cell vesicles after Ag-NP exposure.
2012-09-25