rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells(CELL AND TISSUE ENGINEERING)
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概要
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All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.
著者
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Artmann Aysegul
Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences
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Linder Peter
Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences
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Kayser Peter
Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences
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Porst Dariusz
Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences
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Artmann Gerhard
Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences
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Linder Peter
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Demirci Eylem
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Demirci Taylan
Dokuz Eylul University, School of Medicine, Department of Medical Biology and Genetics
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Trzewik Juergen
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Gierkowski Jessica
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Gossmann Matthias
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Kayser Peter
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Porst Dariusz
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Digel Ilya
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Artmann Gerhard
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Artmann Aysegul
Aachen University of Applied Sciences, Institute of Bioengineering, Laboratory of Cell Biophysics
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Digel Ilya
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Demirci Eylem
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Demirci Taylan
Dokuz Eylul University School Of Medicine Department Of Medical Biology And Genetics
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Trzewik Juergen
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Artmann Aysegul
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Artmann Gerhard
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Gierkowski Jessica
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Gossmann Matthias
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Porst Dariusz
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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Kayser Peter
Aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Cell Biophysics:aachen University Of Applied Sciences Institute Of Bioengineering Laboratory Of Medical And Molecular Biology:aachen University Of Applied Sciences Institute O
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- rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells
- rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells(CELL AND TISSUE ENGINEERING)
- rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells