Oral Lead Exposure Induces Dysbacteriosis in Rats

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概要

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• Objective: Lead's (Pb(II)) possible role in intestinal pathologies of microbial etiology remains mostly unknown. The aim of this study was to examine the effects of lead on the gut microbial community and its interactions with rat intestinal epithelium. Methods: The lead-induced changes in different intestinal microbial groups (lactose-positive lac(+) and -negative lac(-) E. coli strains, lactobacilli and yeasts) were followed separately by the colony-forming unit (CFU) method. Samples were taken from outbred white rats subjected to different exposure schedules. Additionally, the impact of different lead doses on microbial adhesion to cultured intestinal cells (IEC-6) was investigated. Finally, the lead accumulation and distribution were measured by means of atomic absorption spectrometry. Results: For the first time it was shown that oral lead exposure causes drastic changes in the gut microbial community. Proportional to the lead dose received, the relative number of lactose-negative E. coli cells increased dramatically (up to 1,000-fold) in comparison to the other microbial groups during 2wk of exposure. Considering the number of microbes in the intestine, such a shift in intestinal microflora (dysbacteriosis) is very significant. Adhesion studies showed certain stimulating effects of lead on E. coli attachment to rat intestinal epithelium as compared to Lactobacillus attachment. Conclusions: The mechanisms providing the apparent competitive success of the lac(-) group are unclear but could be related to changes in surface interactions between microbial and host cells. This study may provide important clues for understanding the pathological effects of metal dietary toxins in human beings.

• 社団法人日本産業衛生学会の論文

著者

• Digel Ilya

  Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences

• Artmann Aysegul

  Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences

• Linder Peter

  Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences

• Kayser Peter

  Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences

• SADYKOV Rustam

  Kazakh National University Named after Al-Farabi

• PORST Dariusz

  Laboratory of Cellular Biophysics, Aachen University of Applied Sciences

• ARTMANN Gerhard

  Laboratory of Cellular Biophysics, Aachen University of Applied Sciences

• SAVITSKAYA Irina

  Kazakh National University Named after Al-Farabi

• ZHUBANOVA Azhar

  Kazakh National University Named after Al-Farabi

• Porst Dariusz

  Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences

• Artmann Gerhard

  Laboratory Of Cellular Biophysics Aachen University Of Applied Sciences

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