Simultaneous induction of calcium transients in embryoid bodies using microfabricated electrode substrates(CELL AND TISSUE ENGINEERING)
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概要
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Precise control of differentiation processes of pluripotent stem cells is a key component for the further development of regenerative medicine. For this purpose, combining a cell-aggregate-size treatment for regulating intercellular signal transmissions and an electrical stimulation technique for inducing cellular responses is a promising approach. In the present study, we developed microfabricated electrode substrates that allow simultaneous stimulation of embryoid bodies (EBs) of P19 cells. Mouse embryonal carcinoma P19 cells can be induced to differentiate into three germ layers and serve as a promising stem cell model. Microcavity-array patterns were fabricated onto indium-tin-oxide (ITO) substrates using a standard photo-lithography technique, and uniform-sized EBs of P19 cells were inserted into each microcavity. Electrical stimulation was applied to the EBs through substrate electrodes and stimulus-induced intracellular calcium transients were monitored. We confirmed that the developed electrode device could simultaneously stimulate smaller (200μm diameter) and larger (500μm diameter) EBs inserted in the microcavities and induce specific spatio-temporal patterns of intracellular calcium transients in the EBs with fine reproducibility. We concluded that the developed microcavity array with embedded electrodes could simultaneously and effectively stimulate uniform-sized EBs inserted in it. Therefore, it is a promising experimental tool for precisely controlling cell differentiation processes.
著者
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Jimbo Yasuhiko
Department Of Human And Engineered Environmental Studies Graduate School Of Frontier Sciences Univer
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Jimbo Yasuhiko
Graduate School Of Frontier Sciences The Univ. Of Tokyo
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Jinbo Yasuhiko
Graduate School Of Frontier Sciences The University Of Tokyo
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Kotani Kiyoshi
Department Of Human And Engineered Environmental Studies Graduate School Of Frontier Sciences Univer
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Kotani Kiyoshi
Graduate School Of Frontier Sciences The University Of Tokyo
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Saito Atushi
Department Of Human And Engineered Environmental Studies Graduate School Of Frontier Sciences Univer
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Takayama Yuzo
Department Of Human And Engineered Environmental Studies Graduate School Of Frontier Sciences Univer
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Moriguchi Hiroyuki
Department Of Human And Engineered Environmental Studies Graduate School Of Frontier Sciences Univer
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Moriguchi Hiroyuki
Graduate School Of Frontier Sciences University Of Tokyo
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Takayama Yuzo
Graduate School Of Frontier Sciences University Of Tokyo
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Mabuchi Kunihiko
Graduate School Of Information Science And Technology University Of Tokyo
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Saito Atsushi
Japan Society for the Promotion of Science
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Suzuki Takafumi
Graduate School of Information Science and Technology, University of Tokyo
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Suzuki Takafumi
Graduate School Of Information Science And Technology University Of Tokyo
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Moriguchi Hiroyuki
Department Of Human And Engineered Environmental Studies Graduate School Of Frontier Sciences The Un
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Saito Atsushi
Graduate School Of Frontier Sciences University Of Tokyo
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Suzuki Takafumi
Graduate School Of Information Science And Technology The University Of Tokyo
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Mabuchi Kunihiko
Graduate School Of Information Science And Technology The University Of Tokyo
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Takayama Yuzo
Graduate School Of Information Science And Technology University Of Tokyo:the Japan Society For The Promotion Of Science
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