Magnetic purification of plasminogen from human plasma by specific lysine affinity(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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A novel magnetic adsorbent was prepared by covalently binding lysine onto the surface of nanoparticles via a carbodiimide coupling method. The adsorption of plasminogen onto surface-modified magnetic nanoparticles from human plasma was studied in a batch system. Surface modifications of particles were characterized using Fourier-transformed infrared spectra, transmission electron micrography, and ninhydrin assay. The maximum weight ratio of lysine to the superparamagnetic particles was 30μmol/mg of particles. Effects of pH and temperature on plasminogen adsorption by the magnetic particles were evaluated. Desorption of plasminogen from the magnetic adsorbent was investigated using aminocaproic acid, a lysine analogue. Using a buffer composed by aminocaproic acid or lysine, plasminogen on the magnetic nanoparticles could be eluted. Overall, the results demonstrated that the lysine-coated magnetic adsorbent increased the efficiency and speed of recovery of plasminogen from human plasma.
著者
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Liu Chi-hsien
Graduate Inst. Of Biochemical And Biomedical Engineering Chang Gung Univ. Taoyuan Taiwan
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Lai Hsuan-yu
Graduate Institute Of Biochemical And Biomedical Engineering Chang Gung University
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Wu Wei-Chi
College of Medicine, Chang Gung University
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Hou Hau-Yaun
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University
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Wu Wei-chi
College Of Medicine Chang Gung University:department Of Ophthalmology Chang Gung Memorial Hospital
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Hou Hau-yaun
Graduate Institute Of Biochemical And Biomedical Engineering Chang Gung University
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