Promotion of Recombinant Macrophage Colony Stimulating Factor Production by Dimethyl Sulfoxide Addition in Chinese Hamster Ovary Cells(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
A Chinese hamster ovary (CHO) cell line is the primary choice for the production of recombinant protein drugs with glycosylation modification. Recombinant protein productivity was enhanced by the addition of dimethyl sulfoxide (DMSO) in CHO cells. However, DMSO induced G0/G1 phase growth arrest and reduced cell growth rate. A two-stage process was developed to utilize the stimulatory effect of DMSO on recombinant protein production and mitigate the problem of growth inhibition in this study. In the first stage, cells are cultured without DMSO for a certain period in order to obtain a high cell density. Sequentially, DMSO is added to achieve a high specific productivity in the second stage. Using this approach, we found that by adding 1% DMSO after 24h of growth, macrophage colony-stimulating factor (M-CSF) volumetric productivity is increased by 57% compared with the value obtained without the addition of DMSO.
- 社団法人日本生物工学会の論文
- 2007-01-25
著者
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Liu Chi-hsien
Graduate Inst. Of Biochemical And Biomedical Engineering Chang Gung Univ. Taoyuan Taiwan
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Chen Li-Hsin
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University
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Chen Li-hsin
Graduate Institute Of Biochemical And Biomedical Engineering Chang Gung University
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