Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in porous hyaluronic acid scaffold(CELL AND TISSUE ENGINEERING)
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概要
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Human adipose-derived mesenchymal stem cells (AD-MSCs) attracted much interest as a promising alternative to autologous chondrocytes and bone marrow-derived mesenchymal stem cells for cartilage regeneration. Developing a suitable culture technique to direct AD-MSCs into the chondrogenic lineage could be a crucial prerequisite for the cartilage defect repair application of AD-MSCs. Herein, we prepared the PEGDG-crosslinked porous three-dimensional (3D) hyaluronic acid (HA) scaffold and evaluated for its feasibility to induce proliferation and chondrogenic differentiation of the AD-MSCs. In addition, the effect of bone-morphogenetic protein-2 (BMP-2) and platelet-derived growth factor (PDGF) on chondrogenic differentiation was further investigated. Proliferation and chondrogenic differentiation were evaluated by cell morphology, DNA contents, s-GAG contents, and level of mRNA expression of relevant marker genes. When cultured with reference chondrogenic medium (RCM; serum-free DMEM-HG supplemented with 10ng/mL of transforming growth factor-β1 (TGF-β1), 50nM ascorbate, 100nM dexamethasone, and 5μg/mL of ITS), better proliferation and chondrogenic differentiation of AD-MSCs were obtained in the 3D HA scaffold culture as compared to the micromass culture, a standard 3D culture system. Moreover, the level of chondrogenic differentiation of AD-MSCs in the HA scaffold-RCM culture system was further increased by BMP-2, and decreased by PDGF. These results suggested that the HA scaffold with RCM was a promising chondrogenic culture system of AD-MSCs, and that BMP-2 could potentially serve as a chondrogenic supplement for AD-MSCs. However, PDGF was determined to be an inappropriate supplement based on its inhibition of the chondrogenic differentiation of AD-MSCs.
著者
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Sung Jong-hyuk
Prostemics Research Institute Division Of Stem Cell Research
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Shim Chang
Department Of Pharmaceutics College Of Pharmacy Seoul National University
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Chung Suk-jae
Department Of Pharmaceutics College Of Pharmacy Seoul National University
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Chung Suk-jae
College Of Pharmacy Seoul National University
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Kim Jung
Division Of Life Science School Of Life Science And Biotechnology Korea University
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Kim Dae-duk
Department Of Pharmaceutics College Of Pharmacy Seoul National University
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Kim Dae-duk
College Of Pharmacy Chonnam National University
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Shim Chang-koo
College Of Pharmacy Seoul National University
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Chung Suk
Department Of Pharmaceutics College Of Pharmacy Seoul National University
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Chung Suk-jae
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University
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Yoon In-Soo
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
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Chung Chung
Pusan National University Yangsan Hospital
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Sung Jong-Hyuk
Department of Applied Bioscience, CHA University
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Cho Hyun-Jong
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
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Shim Won-Sik
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
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Yoon In-soo
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University
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Shim Won-sik
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University
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Cho Hyun-jong
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University
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Sung Jong-hyuk
Department Of Applied Bioscience Cha University
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Shim Chang-koo
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University
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Kim Dae-duk
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University
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Kim Jung
Division Of Health Science Dongseo University
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