J1102-3-4 金コロイド法を用いた皮膚マーカー分析装置の提案([1102-3]ライフサポート(3))

概要

論文の詳細を見る
Cathepsin L (CatL) has been focusing as a biomarker for evaluating photo-stress. The purpose is to propose an on-site testing method of CatL in skin which can be used for the diagnosis of photo-stress non-invasively, easily, quickly and at any time. In this study, a novel immuno-chromatographic test-strip is proposed for CatL analysis in skin. In order to realize the disposable test-strip, colloidal gold technique was selected as the molecular recognition method of the CatL. The fabricated test-strip consists of a sample pad, a conjugate pad, a nitrocellulose membrane and an absorption pad. After dropping a sample, it required 90 seconds to reach the sample at the test line on the test-strip and indicated a red colored band 3 minutes later. The optical density was proportion to the activity of CatL. It was suggested that the test-strip might be used for the qualitative analysis of CatL in the stratum corneum.
一般社団法人日本機械学会の論文
2010-09-04